Abstract The microbiome is fundamentally one of the most unique organs in the human body.Dysbiosis can result in critical inflammatory responses and result in pathogenesis contributing to neoplastic events.We used a pan-pathogen array technology (PathoChip) coupled with next-generation sequencing to establish microbial signatures unique to human oral and Jump Rope oropharyngeal squamous cell carcinomas (OCSCC/OPSCC).Signatures for DNA and RNA viruses including oncogenic viruses, gram positive and negative bacteria, fungi and parasites were detected.Cluster and topological analyses identified 2 distinct groups of microbial signatures related to OCSCCs/OPSCCs.
Results were validated by probe capture next generation sequencing; the data from which also provided a comprehensive map of integration sites and chromosomal hotspots for Leather Biker Jacket micro-organism genomic insertions.Identification of these microbial signatures and their integration sites may provide biomarkers for OCSCC/OPSCC diagnosis and prognosis as well as novel avenues for study of their potential role in OCSCCs/OPSCCs.